Effectiveness Test of Anti-Inflammation of Ethanol-Extracted Cream of Graptophyllum pictum L. Griff On White Male Mice (Mus musculus)

摘要

Background: Graptophyllum pictum L. Griff are commonly used in traditional medications. One of them is as an anti-inflammation. Giving medicine topically may increase bioavability and medicine efficacy and reduce side effects of conventional anti-inflammatory medicine. The ethanol extract of black pudding leaves had been formulated into cream form. The aim of this study is to investigate the effectiveness of the cream-formed anti-inflammation of ethanol extract of Graptophyllum pictum L) Griff on white male mice (Mus musculus).Purpose: The effectiveness of anti-inflammation was tested by using paw edema method. The tested animals used were 25 white male mice divided into 5 groups, namely negative control group (F0), positive control group (hydrocortisone cream), and treatment group of ethanol-extracted cream of black pudding leaves F1 (10%), F2 (15%), and F3 (20%). The initial paw volume measurement was done on each mouse, and the treatment for each group was administered after inflammatory induction using 0.1 ml of 1% carrageenan solution. The observation of inflammation decrease on each mouse was carried out every 1 hour during 6 hours using plesthysmometer.Result: The data obtained were then analyzed by using one-way ANOVA statistical test followed by Duncan test with 99% confidence level. The result of one-way ANOVA test showed that there was significant difference between treatment groups (P0.01). Duncan test result showed that the groups that gave the best anti-inflammatory effectiveness were the positive control group and F3 which contained 20% extract concentration.Conclusion: Ethanol-extracted cream of Graptophylum pictum L. Griff gave an anti-inflammatory effect to the tested animals white male mice that had been induced inflammation using carrageenan.