Determination of Imazamox and Imazethapyr Herbicide Residues in Soybean oil

摘要

A easy, sensitive and inexpensive method was evolved the usage of solid-phase extraction, together with high performance liquid chromatographic method with UV detection for determination of imazamox and imazethapyr residues in soybean oil samples. The evaluated parameters consist of the extracts via C18 cartridge, using hydrochloric acid, hexane, methanol, methylene chloride and acetonitrile solvents. The method becomes established the use of soybean oil samples spiked with imazamox and imazethapyr at different fortification levels (0.01 and 0.1 μg/mL). Average recoveries (using each concentration six replicates) ranged 87-94%, with relative standard deviations less than 3%, calibration solutions concentration in the range 0.01-5.0 μg/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.003μg/mL and 0.01μg/mL respectively.