Isolation and Identification of Metal Lead (Pb) Ressistent Microalgae on Branckish Water at Muaro Panjalinan Tabing, Padang, West Sumatera

摘要

This research was done to obtain microalgae isolate which comes from West Sumatera that is Metal Lead (Pb) ressistent and knowing the efficiency and absorption capacity of Pb Metal from Microalgae Isolate. The microalgae sample was taken from branckish water in Muara Panjalinan, Padang, West Sumatera. Isolation was done by using 25 mg/L Pb metal. The isolate identification was done microscopically and mollecularly. On molleculare identification, 18S rRNA was amplified by using PCR with primary: forward (5’-CCTGGTTGATCCTGCCAG-3’) and reverse (5’-TTGATCCTTCTGCAGGTTCA-3’). The Pb absorption efficiency and capacity was analyzed by using Atomic Absorption Spectrophotometer (AAS). The growth of microalgae base on absorption value from spectrophotometer equipment. Based on the result of this research, can be concluded that in Branckish Water at Muara Panjalinan, Padang, West Sumatera, Ochromonas vasocystis microalgae that is ressistent towards heavy metal (Pb 25 mg/L) exists. Based on morphology and mollecular data, the isolate that was isolated can be identified as Ochromonas vasocystis and during the treatment of Pb metal towards microalgae isolated culture, cause the decrease of cell density which inhibit the growth of cell. Beside, absorption capacity and efficiency of metal tends to increase along with the increase of Pb initial concentration. Maximum absorption capacity and efficiency happens at the incubation time of 360 min.