Association of G-2548A Polymorphism in the Promoter of Leptin Gene with Plasma Leptin Level and Risk of Type 2 Diabetes

摘要

Introduction: Leptin, the obese (ob) gene product, is a cytokine-like hormone secreted mainly from adipose tissue acting on a receptor site in the hypothalamus to inhibit food intake and stimulate energy expenditure. A G-2548G polymorphism in the leptin gene promoter has a strong influence on leptin gene expression and adipose tissue secretion. The aim of this study was to examine the association of the leptin G-2548A promoter polymorphism with leptin plasma level and susceptibility to type 2 diabetes.Methods: 100 patients with type 2 diabetes mellitus and 100 healthy controls were screened for the presence of G-2548A polymorphism using PCR-RFLP analysis. Body mass index, fasting leptin and fasting glucose were also determined.Results: Carriers with the GG genotype (20.02±5.6μg/L) had significantly (P