Diagnostic approach in canine parvovirus infection in vaccinated and non-vaccinated dogs.

摘要

The significance of Canine parvovirus 2 (CPV-2) infection for the domesticated canides is well understood. The current study comparesthe morbidity rate and clinical outcome in a group of vaccinated and non-vaccinated dogs (11 and 27 individuals, respectively) presentedfor treatment between October 2009 and March 2011. Fecal samples from the dogs with clinical signs of Canine Parvovirosis (CPV) were testedusing a rapid test based on detection of CPV antigens, and a polymerase chain reaction (PCR) was applied for detection of viral DNA. Forfurther confirmation two of the positive samples were sequenced, and after alignment with the Basic Local Alignment Search Tool (BLAST)they showed over 99% of overlapping with the data in GenBank. There wasn’t significant difference in morbidity between vaccinated and nonvaccinateddogs, but the recovery period was shorter for the first group (mean value 7 days+/-1d, versus 12 days+/-2d). Some of the dogs werevaccinated twice or even thrice, but fail to resist, which might be due to an inadequate vaccination scheme or vaccine, or an early exposureto the wild virus. The latter suggests the increasing need of a modern diagnostic approach, for discrimination between the wild virus and thestrains used for vaccination.