Cationic Lipsomes Promote in Vivo Transfection, Innate Immunity Activation and Antigen-Specific CD8+ T-Cell Activation Following Vaccination with Piggybac DNA Plasmids

摘要

DNA vaccination with plasmid has conventionally involved vectors designed for transient expression of antigen in injected tissues. Next generation plasmids are being developed for site-directed integration of transgenes into safe sites in host genomes and may provide an innovative approach for stable and sustained expression of antigens for vaccination. In our previous study, we have demonstrated the improved efficacy of site-directed integration using hyperactive piggyBac transposase-based integrating vectors (pmhyGENIE-3) over non-integrating plasmids. Adjuvant technologies such as AdjuplexTM may further improve vaccine efficacy by producing a strong immunostimulatory effect to elicit more potent immune responses via both cell-mediated and antibody-mediated mechanisms.