ORF1a of highly pathogenic PRRS attenuated vaccine virus plays a key role in neutralizing antibody induction in piglets and virus neutralization in vitro

摘要

Background Currently, porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important viral pathogens in swine in most countries, especially China. Two PRRSV attenuated live vaccine strains (HuN4-F112 and CH-1R) are currently widely used in China. Our previous study showed that HuN4-F112, but not CH-1R, induced high anti-nucleocapsid (N) antibody and neutralizing antibody (NA) titers. Additionally, sera from HuN4-F112 inoculated pigs induced low cross neutralization of CH-1R. Methods In the present study, 6 chimeric viruses through exchanging 5′ untranslated region (UTR)?+?open reading frame (ORF)1a, ORF1b, and ORF2–7?+?3’UTR between HuN4-F112 and CH-1R were constructed and rescued based on the infectious clones of rHuN4-F112 and rCH-1R. The characteristics of these viruses were investigated in vitro and vivo. Results All the three fragments, 5’UTR?+?ORF1a, ORF1b, and ORF2–7?+?3’UTR, could affect the replication efficiencies of rHuN4-F112 and rCH-1R in vitro. Additionally, both 5’UTR?+?ORF1a and ORF2–7?+?3’UTR affected the anti-N antibody and NA responses targeting rHuN4-F112 and rCH-1R in piglets. Conclusions The 5’UTR?+?ORF1a region of HuN4-F112 played a key role in inducing NAs in piglets. Furthermore, we confirmed for the first time that ORF1a contains a neutralization region. This study provides important information that can be used for further study of the generation of anti-PRRSV NAs.