Monoclonal antibody differentiation between bovine herpesviruses type 1 and 5

摘要

.- Roehe P.M., Silva T.C., Nardi N.B., Oliveira L.G. & Rosa J.C.A. 1997. [Monoclonal antibody differentiation between bovine herpesviruses type 1 and 5.] Diferencia?§?￡o entre os v?-rus da rinotraque?-te infecciosa bovina (BHV-1) e herpesv?-rus da encefalite bovina (BHV-5) com anticorpos monoclonais. Pesquisa Veterin??ria Brasileira 17(1):41-44. Centro de Pesquisas Veterin??rias Desid??rio Finamor, Caixa Postal 2076, Porto Alegre, RS 90001-970, Brazil. Bovine Herpesviruses (BHV) type 1 (BHV-1) and type 5 (BHV-5) were analysed by immunoperoxidase staining with a panel of monoclonal antibodies (Mabs) prepared against BHV antigens. One of the Mabs recognized all BHV isolates tested. The remainder four mabs recognized only BHV-1 samples, including standard laboratory strains. All isolates associated with clinical cases of encephalitis (BHV-5) displayed a pattern of reactivity distinct from that of viruses isolated from syndromes associated with BHV-1 infections. The results obtained indicate that such Mabs allowed the differentiation between BHV-1 and BHV-5, with a perfect correlation between the clinical pictures and the patterns of reactivity in vitro. INDEX