Biological and Histological Studies of Purified Product from Streptomyces janthinus M7 Metabolites

摘要

Fifteen clinical samples were taken out from patients suffering cancer, these patients being under the treatment with radio- and/or chemotherapy. The samples were used for the isolation of bacterial cells surrounding tumor; the samples were collected from Center of Cancer Therapy, Ain Shams University, Cairo, Egypt. The clinical bacterial isolates were purified and identified according to Bergey's manual of determinative bacteriology ninth edition (1994). The bacterial isolates were found to be Klebsiella oxytoca m1; Enterobacter cancerogenus m2; P . aeruginosa m3; Citrobacter diversus m4; Enterobacter agglomerans m5; Klebsiella oxytoca m6; Enterobacter dissolvens m7; Serratia fonticola m8; Escherichia coli m9; Citrobacter freundii m10; Staphylococcus aureus m11; Escherichia coli m12; P . aeruginosa m13; Staphylococcus aureus m14; and Bacillus cereus m15. In the present study both primary and secondary screening methods were used to screen the antibacterial activity of St. janthinus M7 against fifteen clinical bacterial isolates. The St. janthinus M7 showed an increase in antibacterial activity against all the tested human bacterial pathogens. In this study Gamma irradiation at dose levels (0.5 and 1.5 kGy) was used for the enhancement of the antibacterial activity of Streptomyces strain against the clinical isolates. Several commercial antibiotic discs (Doxorubicin, Augmentin, Norfloxacin, Ofloxacin, Oxacillin, and Cefazolin) were used for comparing their antimicrobial activity with purified product. The results declared a significant increase in the antibacterial activity in most cases. The physiochemical properties of the purified product were carried out for determination of R _f , empirical formula, M.W, and chemical structure of product and then analyzed by thin layer chromatography, elemental analysis, UV, Mass, and NMR. The result exhibited brown color, one spot, R _f (0.76), M.W (473), while it recorded 270 nm in UV region and the calculated empirical formula was (C₂₂H₁₉NS₂O₇). In in vitro antitumor activity of the purified product against human tumor cell lines (Hepg2) the IC₅₀ was measured to be 3.0 μ g/ml and in in vitro antitumor activity of purified product measured against Ehrlich ascites carcinoma (EAC) the IC₅₀ was measured to be 33.0 μ g/ml. In in vivo study of the cytotoxic effect of St. janthinus M7 purified product MYN7 was investigated. The result of histopathological alteration in female mice infected with Ehrlich tumor showed a significant effect on abnormal cells.