Effects of Ovaprim Administration on Reproductive Parameters of Shirbot, Barbus grypus , Cyprinidae

摘要

In the present study, we have investigated the inducing effect of ovaprim on reproductive parameters of Shirbot, Barbus grypus including: spawning rate, working fecundity, egg weight/g.bw, egg hatching percent and larval survival rate in order to found an alternative for carp pituitary extract. For this purpose, 105 female fish were divided in 6 experimental treatments and injected intraperitoneally in two stages (first stage by 10% hormone and after 12 hours the second s0% hormone) with different doses of ovtage with 9aprim including: T₁: 0.25 ml/kg.bw, T₂: 0.5 ml/kg.bw, T₃: 0.75 ml/kg.bw, T₄: 1 ml/kg.bw, T₅: 1.25 ml/kg.bw, T₆: 1. 5 ml/kg.bw. Also, one group was considered as control and its fish were injected by 3 mg/kg.bw pituitary extract. To obtain spermatozoa, 3 maturing male fish were injected intraperitoneally by 3/kg.bw pituitary extract in one stage. According to our results, fish of T₁, T₂ and T₆ did not respond to injection and thus spawning did not occur. The highest values of spawning rate, egg weight/g.bw and working fecundity were observed in T₄ and control group. The lower values of spawning rate, egg weight/g.bw and working fecundity were obtained in T₅ compared to other experimental groups. As well as, there were no significant differences between experimental groups in terms of fertilization percent, hatching percent and larval survival rate. The results of this study suggest that a dose of 1 ml/kg.bw ovaprim and 3 mg/kg.bw pituitary extract have better and similar effects on some reproductive parameters of Shirbot such as spawning rate, egg weight/g.bw and working fecundity. Thus, use of traditional method i.e. pituitary extract could be useful yet to induce the reproduction in Shirbot.