Micropropagation of Coelogyne stricta (D.Don) Schltr. Via pseudobulb segment cultures

摘要

Reproducible protocols for micropropagation of Coelogyne stricta via pseudobulb segments were developed. In vitro grown pseudobulb segments responded positively by induction shoot buds on half strength Murashige and Skoog?s (MS) medium supplemented with growth regulators α-naphthalene acetic acid (NAA) and Benzyl Adenine (BA) individually and in combinations. The combination of NAA (1.0 mg/l) + BA (2.0mg/l) and BA individually induced maximum number of multiple shoots. Root number proved to increase with NAA (1.0 and 2.0 mg/l) in the MS medium. The well developed rooted plantlets were hardened successfully in the potting mixture containing coconut husk, perlite, charcoal, broken tiles, brick pieces in the ratio of 2:1:1:1:1.