Biochemical, rheological and hydrophile-lipophile balance (HLB) evaluation of Archachatina marginata (snail) mucin extract for possible nutraceutical and nano biopharmaceutical applications

摘要

Purpose: To evaluate the rheological, biochemical, hydrophile-lipophile balance (HLB) of Archachatina marginata (snail) mucin extract for possible use as a nutraceutical and nano biopharmaceutical material. Methods: Snail mucin was extracted with acetone and water, lyophilized and the biochemical, proximate and mineral analyses of the extracts were studied using standard methods. The rheological properties of the extracts (1, 2, 4 and 8 % w/v) and their emulsion-based preparations were evaluated. Other physicochemical properties and HLB values of the preparations were also determined. Results: Snail mucin extracts contained protein (84 %), fats (2.91 %) and carbohydrate (1.2 %) and showed significant nutraceutical composition (p 0.05). Ash content of 4.21 and 4.12 % was obtained for water and acetone extracts, respectively. Moisture content was 9 % for both the aqueous and non-aqueous mucin extracts. Potassium, calcium and phosphorus were present in high quantities in the extracts while iron, copper and zinc were in trace amounts ( 4 %). Mucin dispersions exhibited viscosity in the range of 0.89 to 0.93 cp. Water sorption and dry weight were higher in the acetone extract than in the aqueous extract. The HLB values, which ranged from 7 to 15, were within the acceptable values for material for nanobiopharmaceutical application, except that the acetone extract. Conclusion: Snail mucin exhibits good nutraceutical properties and also possesses good properties that render it a potential excipient for use in the formulation of drug delivery systems.