Cardioprotective effect of melatonin-standardized ethanol extract of Ulva lactuca L against acute myocardial infarction in rats induced by isoproterenol

摘要

Purpose: To investigate the cardioprotective effect of standardized melatonin ethanol extract of Ulva lactuca (smEEUL) against acute myocardial infarction induced by isoproterenol (ISO). Methods: Male Wistar rats were divided into 6 groups of 6 rats each. Group A was control group given carboxymethylcellulose (CMC Na) while Group B was given ISO 85 mg/kg BW, subcutaneously. Groups C, D and E were given ISO plus smEEUL 250, 500 and 750 mg/kg orally, respectively. Group F was given melatonin 10 mg/kg. Both smEEUL and melatonin were administered for 28 days. ISO was injected twice, i.e., on days 29 and 30. At the end of the study, electrocardiogram (ECG) was recorded, and blood samples collected for myocardial necrosis enzyme assay. Macroscopic features of left ventricle of the heart were also examined using triphenyl tetrazolium chloride (TTC) staining Results: ISO-treated group demonstrated a significant (p Conclusion: The results show that administration of smEEUL produces cardioprotective effect by restoring ST segment elevation, and reducing both the area of myocardial infarction and myocardial necrosis enzyme level.