Polysaccharide extracts of iCirsium japonicum/i protect rat H9c2 myocardial cells from oxidative stress induced by hydrogen peroxide

摘要

Highlights The current study found that the polysaccharide extract of Daji (Cirsium japonicum) have a favorable therapeutic effect against oxidative stress and the underlying mechanism might be involved with MAPK pathway. Editor’s Summary Daji (Cirsium japonicum) have been traditionally utilized by the Tibetan and Mongolian people since the Tang Dynasty of China (618 A.D. - 907 A.D.). Daji (Cirsium japonicum) has been applied against gastric disorders, lung diseases, and cardiovascular problems in the traditional Chinese medicinal system. The present study was to investigate the protective effects of Daji (Cirsium japonicum) polysaccharide extracts (CJP) against hydrogen peroxide (H2O2) shock in rat H9c2 myocardial cells. First, CJP was isolated by hot water extraction and ethanol precipitation; it was then characterized by high performance liquid chromatography and infrared spectrum analysis. Rat H9c2 cells were subjected to H2O2 treatment to establish a cell injury model. The 3- (4,5- dimethylthiazol- 2-yl)-2,5- diphenyltetrazolium bromide assay showed that CJP pretreatment significantly ameliorated the H2O2 injury in a dose-dependent manner. Furthermore, the cell apoptosis induced by H2O2 was markedly inhibited by CJP pretreatment, whereas the cleavage level of caspase-3, -8, and -9 was reduced. In addition, the p38 mitogen-activated protein kinase pathway might be involved in the protective effect of CJP on myocardial cells. Therefore, we conclude that polysaccharide extracts of Daji (Cirsium japonicum) protect rat H9c2 myocardial cells from oxidative stress induced by H2O2.