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首页> 外文期刊>Toxicology Reports >Safety effect of fractions from methanolic leaf extract of Ocimum gratissimum on reproduction in male wistar rats
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Safety effect of fractions from methanolic leaf extract of Ocimum gratissimum on reproduction in male wistar rats

机译:洋地黄的甲醇叶提取物馏分对雄性Wistar大鼠生殖的安全性影响

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摘要

This study evaluates the reproductive toxicity of ethyl acetate and butanolic fractions from crude methanolic leaf extract of Ocimum gratissimum in male Wistar rats. Acute toxicity was assessed to determine the safety dose, Sub-chronic reproductive toxicity studies were carried out by administering daily 25, 100 and 400?mg/kg body weight doses of the fractions to respective group of animals and 1?ml of normal saline daily for the control group for 28 days. Blood, epididymis and testes were harvested for reproductive hormones, sperm parameters, and histopathologic analysis respectively. There was significant (P??0.05) increase in serum levels of testosterone, body-weight gain, sperm count. There was also apparent increase in mean-testicular weight and preservation of testicular histology with increase spermatogenesis in both the ethyl acetate and butanolic fraction treated groups compared with control. Serum levels of luteinising hormone was however significantly (P??0.05) decrease across the groups compared to control. These effects were more pronounced in the butanolic fraction group compared to the ethyl acetate treated group. Sperm motility was also significantly (P??0.05) higher in the ethyl acetate treated group compared to control. Findings from this studies demonstrate that these fractions were non-toxic at the tested doses with regards to male reproduction but, rather, exhibited fertility enhancing effects which was better with the butanolic fraction. Our findings also shows that the ethyl acetate fraction may be safer than the butanolic fraction.
机译:这项研究评估了雄性Wistar大鼠从粗制无齿O的甲醇叶提取物中乙酸乙酯和丁醇馏分的生殖毒性。通过评估急性毒性来确定安全剂量,通过分别向各组动物和分别每天分别给予25、100和400?mg / kg体重级分的级分进行亚慢性生殖毒性研究。对照组为28天。分别采集血液,附睾和睾丸进行生殖激素,精子参数和组织病理学分析。血清睾丸激素,体重增加,精子数量显着增加(P <0.05)。与对照组相比,乙酸乙酯和丁醇馏分处理组的平均睾丸重量明显增加,睾丸组织学得以保留,同时精子发生增加。然而,与对照组相比,各组的血清黄体激素水平显着降低(P≤0.05)。与乙酸乙酯处理的组相比,这些作用在丁醇级分组中更为明显。与对照组相比,乙酸乙酯处理组的精子运动能力也显着提高(P≤0.05)。这项研究的结果表明,这些馏分在测试剂量下对雄性生殖无毒,但是表现出增强生育力的作用,而丁醇馏分则更好。我们的发现还表明,乙酸乙酯级分可能比丁醇级分更安全。

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