Improvement in Sensitivity of Live Cell-Based Sensor Cells by Co-Transfection of a Reporter Gene Driven by a Modified HSP70B' and HSF1Expression Vector

摘要

We previously reported that live cells transfected with a luciferase construct carrying the HSP70B' gene promoterincreases the luciferase activity in response to toxic reagents and can be used as an intelligent cytotoxicity sensordevice (i.e., sensor cells). In the present study, in order to improve the sensitivity of these cytotoxic sensor cells, we cotransfectedthe sensor cells with both the luciferase construct carrying the HSP70B' gene promoter (pMCREx3) and anHSF1 expression vector driven by a modified HSP70B’ promoter (pCRE/HSF). Cells co-transfected with pMCREx3 andpCRE/HSF showed 2.5 times higher sensitivity for CdCl2 cytotoxicity than cells transfected with pMCREx3 alone, indicatingthat sensor cells co-transfected in this way are useful and sensitive tools with which to detect a cytotoxic response.