Indexing Tools for Indian Citrus Ringspot Virus (ICRSV)

摘要

Indian citrus ringspot virus (ICRSV) is known to cause serious disease problem in Kinnow (Citrus nobilisLourC. deliciosa Tenora). This paper reports the various methods viz. Bioassay, ELISA and RT-PCR for indexing ofICRSV. Bioassay was performed on Chenopodium amaranticolor, Cucumis sativus, Nicotiana glutinosa, N. tabacum, Petuniahybrida and Phaseolus vulgaris. However necrotic local lesions were observed only in case of Chenopodium amaranticolorand Phaseolus vulgaris. Infected trees were also found positive by indirect ELISA. RT-PCR of the infectedplants showed an amplification of 539 bp fragment corresponding to coat protein gene and gene for nucleic acid bindingprotein.