Sequential Use of Fibroblastic Growth Factor 2 and Transforming Growth Factor β1 in Cartilage Tissue Engineering Using Canine Chondrocytes

Wanda J.Gordon-Evans; Michael J. Yaeger; M. Heather Greenlee; Michael G. Conzemius

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Sequential Use of Fibroblastic Growth Factor 2 and Transforming Growth Factor β1 in Cartilage Tissue Engineering Using Canine Chondrocytes

机译：成纤维细胞生长因子2和转化生长因子β1在犬软骨细胞软骨组织工程中的顺序使用

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This study investigated the effect of fibroblast growth factor 2 in monolayer followed by transforming growthfactor β1 in 3-dimensional culture on chondrocytic matrix production. Under the culture conditions presented, FGF2 ismitogenic but not chondrogenic. In addition, TGFβ1 stimulation produced predominantly type I collagen.

机译：本研究调查了成纤维细胞生长因子2在单层中，随后在3维培养中转化生长因子β1对软骨细胞基质产生的影响。在呈现的培养条件下，FGF2具有促成丝性，但不具有软骨生成性。另外，TGFβ1刺激主要产生I型胶原。

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《The Open Tissue Engineering and Regenerative Medicine Journal》 |2008年第1期|共6页
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Wanda J.Gordon-Evans; Michael J. Yaeger; M. Heather Greenlee; Michael G. Conzemius;
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中图分类生物学;
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机译：成纤维细胞生长因子和转化生长因子-β对猪软骨细胞和组织工程化的自体软骨的影响。
2. Advancing osteochondral tissue engineering: bone morphogenetic protein, transforming growth factor, and fibroblast growth factor signaling drive ordered differentiation of periosteal cells resulting in stable cartilage and bone formation in vivo [J] . L. F. Mendes, H. Katagiri, W. L. Tam, Stem Cell Research & Therapy . 2018,第1期

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3. Interaction of chondrocytes, extracellular matrix and growth factors: relevance for articular cartilage tissue engineering. [J] . van der Kraan PM, Buma P, van Kuppevelt T, Osteoarthritis and cartilage . 2002,第8期

机译：软骨细胞，细胞外基质和生长因子的相互作用：与关节软骨组织工程的相关性。
4. Covalently Tethered Transforming Growth Factor Beta-1 in PEG Hydrogels Expedites Cartilage ECM Production of Encapsulated Primary Chondrocytes [C] . Balaji V. Sridhar, Nicholas R. Doyle, Kristi S. Anseth Society for Biomaterials annual meeting and exposition . 2013

机译：PEG水凝胶中的共价束缚的转化生长因子Beta-1加快了封装的原代软骨细胞的软骨ECM生产。
5. Src kinase signaling regulates connective tissue growth factor (CTGF/CCN2) induction by transforming growth factor-beta 1 (TGF-beta1) in osteoblasts. [D] . Zhang, Xuemei. 2010

机译：Src激酶信号传导通过转化成骨细胞中的生长因子-beta 1（TGF-beta1）来调节结缔组织生长因子（CTGF / CCN2）的诱导。
6. Advancing osteochondral tissue engineering: bone morphogenetic protein transforming growth factor and fibroblast growth factor signaling drive ordered differentiation of periosteal cells resulting in stable cartilage and bone formation in vivo [O] . L. F. Mendes, H. Katagiri, W. L. Tam, 2018

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7. PA70 Differential regulation of connective tissue growth factor mRNA levels by transforming growth factor-P and tumour necrosis factor-α in canine articular chondrocytes [O] . 2001

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8. Use of fibroblast growth factor 2 for expansion of chondrocytes and tissue engineering [R] . 2003

机译：使用成纤维细胞生长因子2扩增软骨细胞和组织工程

Sequential Use of Fibroblastic Growth Factor 2 and Transforming Growth Factor β1 in Cartilage Tissue Engineering Using Canine Chondrocytes

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