In Vitro Micrografting of Mature Carob Tree (Ceratonia siliqua L.)

摘要

A micrografting technique was developed for Ceratonia Siliqua using in vitro germinated seedlings as root-stocksand apex or shoot cultures (established from mature female tree source) as microscions. In vitro germinated seedlingswere decapitated and used as rootstock. Mature explants were initiated on Murashige and Skoog medium containing30 g/l sucrose, and 0.5 mg/l 6-benzylaminopurine, then transferred on the multiplication medium (Murashige and Skoog +0.5 mg/l 6-benzylaminopurine + 0.1 mg/l gibberellic acid + 0.1 mg/l indol-3- butyric acid). Micrografts could be easilycultured on the same medium and developed after one month. Grafting success has been dependent on the method ofgrafting. The micrografting on hypocotyl (central grafting) method was significantly more successful (92, 50%) than theother grafts methods. The compatible graft union was observed one month after grafting. The histological cuttings showedthat the graft union formation was initiated by the development of the callus bridge at the interface between scion androotstock, after that, new vascular elements including vessels and tracheids were seen across the interface zone.