Target Site Preferences of Subgroup C Rous Sarcoma Virus Integration into the Chicken DNA

摘要

We sequenced unselected integration sites of subgroup C Rous sarcoma virus from infected chicken cells andmapped them on the chicken draft genome assembly. Our genome-wide analysis demonstrates the non-random characterof subgroup C Rous sarcoma virus integration into genes, gene-rich regions and GC-rich regions. Within genes, there isno significant integration bias in favor of transcription start sites. Integration sites are underrepresented on microchromosomes.These results may be important for the development of gene transfer vectors and gene therapy strategies based onavian sarcoma and leukosis viruses.