Comparative Nucleotide Sequence of Encoding for Quaking Protein of Birds and Mammals

摘要

Partial nucleotide sequences of eight kinds of bird (common crane, gray pelican, goose, black swan, bewisk’sswan, whooper swan, ostrich, and chicken) gene encoding quaking protein were analyzed. Variously sized bands were observedin all eight birds compared with the same sized band in mammals based on nested PCR results, and all sequencedata showed a very high level of homology. Three bases (CD223(Ala)) in exon 6 of all eight bird species were defectivecompared with sequence data from the mouse, and both the existence and defect of 24 bases (CD211 ~ 219) in exon 6 ofthe eight birds were confirmed on complementary DNA sequence analysis. These findings suggest that birds generatespecific alternative sequencing data, there are many alternative splicing variants, and the avian quaking gene may be aspecific marker differentiating between birds and mammals.