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首页> 外文期刊>The Journal of Veterinary Medical Science >Immunoperoxidase Procedures for Rapid Detection of Bovine Viral Diarrhea-Mucosal Disease Virus Antigen
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Immunoperoxidase Procedures for Rapid Detection of Bovine Viral Diarrhea-Mucosal Disease Virus Antigen

机译:免疫过氧化物酶程序,用于快速检测牛病毒性腹泻-粘膜病病毒抗原

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摘要

References(12) Cited-By(2) Bovine viral diarrhea-mucosal disease (BVD-MD) virus antigen was detected in bovine fetal muscular cells from sera of 42 cattle persistently infected with noncytopathic BVD-MD virus by the indirect immunoperoxidase procedure (IIP) but not from sera of 100 apparently healthy cattle. These findings agreed with the results obtained by the interference method. Furthermore, BVD-MD virus antigens were detectable in the smears of buffy coats by the IIP procedure and in the formalin-fixed tissue sections by the avidin-biotin-peroxidase complex procedure. These procedures are therefore expected to be useful for rapid detection of BVD-MD virus antigen.
机译:参考文献(12)被引用的By(2)通过间接免疫过氧化物酶方法(IIP)在42例持续感染非细胞病性BVD-MD病毒的牛血清中的牛胎儿肌肉细胞中检测到牛病毒性腹泻-粘膜病(BVD-MD)病毒抗原),但不是来自100头看上去健康的牛的血清。这些发现与干涉法获得的结果一致。此外,BVD-MD病毒抗原可通过IIP方法在血沉棕黄层的涂片中检测,而通过抗生物素蛋白-生物素-过氧化物酶复合物方法可在福尔马林固定的组织切片中检测到。因此,这些方法有望用于快速检测BVD-MD病毒抗原。

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