Bacillus shacheensis sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil

摘要

A moderately halophilic bacterium, strain HNA-14T, was isolated from a saline-alkali soil sample collected in Shache County, Xinjiang Province. On the basis of the polyphasic taxonomic data, the isolate was considered to be a member of the genus Bacillus. The organism grew optimally at 30?°C and pH 8.0. It was moderately halophilic and its optimum growth occurred at 5a?’10% NaCl. The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid and the predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C15:0 and iso-C15:0 and the polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and two unknown phospholipids. The G+C content of the genomic DNA was 48.6 mol%. Strain HNA-14T exhibited a low 16S rRNA gene sequence similarity of 96% with its nearest neighbors [Bacillus clausii KSM-K16 (96.5%), Bacillus xiaoxiensis DSM 21943T(96.2%), Bacillus clausii DSM 8716T (96.1%), Bacillus patagoniensis PAT05T (96.1%), Bacillus lehensis MLB-2T (96.0%), Bacillus oshimensis K11T (95.9%) and Bacillus hunanensis DSM 23008T (95.8%)] and the phenotypic characteristics indicate that strain HNA-14T can be distinguished from them. Therefore, a novel species of the genus Bacillus, Bacillus shacheensis sp. nov. (type strain, HNA-14T=KCTC 33145=DSM 26902) is proposed.