Optimization of phytoremediation in Cd- contaminated soil by using Taguchi method in Spinacia oleracea

摘要

Phytoremediation is an environmental friendly technique to the cleanup of polluted soils, which combines the disciplines of plant, soil and microbiology. In this study, four factors including: cow manure, compost, urea fertilizer and Cd-resistant bacteria with three different levels in soils contaminated with cadmium using 50 mg kg-1 cadmium chloride (CdCl2.H2O) were used to optimize of phytoremediation by Spinacia oleracea. Taguchi method has been used for experimental design. Results showed that significant factors in the order of importance were: cow manure, Cd- resistant batteries, urea fertilizer and compost. The optimum conditions for the selected levels were inoculate three types of bacteria (CC3, CC4, CC5), compost = 10 (g kg-1), urea fertilizer = 0.05 (g kg-1) and cow manure = 40 (g kg-1). The performance of` these conditions were estimated at 257.27 (mg kg-1). Cow manure is the most contribution to efficiency of phytoremediation in Spinacia oleracea.