Laboratory mouse housing conditions can be improved using common environmental enrichment without compromising data

摘要

Animal welfare requires the adequate housing of animals to ensure health and well-being. The application of environmental enrichment is a way to improve the well-being of laboratory animals. However, it is important to know whether these enrichment items can be incorporated in experimental mouse husbandry without creating a divide between past and future experimental results. Previous small-scale studies have been inconsistent throughout the literature, and it is not yet completely understood whether and how enrichment might endanger comparability of results of scientific experiments. Here, we measured the effect on means and variability of 164 physiological parameters in 3 conditions: with nesting material with or without a shelter, comparing these 2 conditions to a “barren” regime without any enrichments. We studied a total of 360 mice from each of 2 mouse strains (C57BL/6NTac and DBA/2NCrl) and both sexes for each of the 3 conditions. Our study indicates that enrichment affects the mean values of some of the 164 parameters with no consistent effects on variability. However, the influence of enrichment appears negligible compared to the effects of other influencing factors. Therefore, nesting material and shelters may be used to improve animal welfare without impairment of experimental outcome or loss of comparability to previous data collected under barren housing conditions. Author summary Adequate housing of laboratory animals is essential to guarantee their well-being. From a scientific perspective, physically and mentally healthy animals also contribute to increased validity and reproducibility of experimental results. The choice of nesting material or shelter type, referred to as environmental enrichment, may influence how laboratory animals perform species-specific behaviors. Consequently, changes in these nesting and shelter materials could influence scientific results by, for example, increasing variability in measured characteristics. Whether studies using different environmental enrichment materials can be compared is currently questioned. Our study shows that simple, species-specific environmental enrichment in the form of nesting material alone or in combination with a shelter did not consistently increase variability of physiological parameters in mice. Differences in parameter average values appeared to be of minor biological relevance when compared to the effects of other environmental factors. These simple environmental enrichment devices may therefore be applied to improve the housing environment of laboratory mice without compromising data validity or comparability.