In-silico approaches in comparative genomics, structure prediction and functional characterization of secondary metabolite proteins of Mentha sp.

摘要

Mentha species are the main source of a number of natural aroma chemicals. In this paper, an attempt has been made to study some important Mentha species, viz., M. piperita, M. canadensis and M. arvensis. In-silico approaches were used for EST assembly, comparative genomics, homology modeling, molecular threading, domain and fold recognition, secondary structure analysis, physicochemical and functional characterization. Totally, 164 different proteins were reported from contigs which were subjected to modeling. Detailed functional and structural characterizations were carried out for 53 different secondary metabolite proteins of Mentha. Physicochemical characterization of the proteins revealed their aliphatic index instability index and extinction coefficient values. These findings will help in further annotation of Mentha from EST level to protein level.Pages 354-363 |