Molecular and phylogenetic studies of Colletotrichum truncatum associated with soybean anthracnose in India

摘要

Colletotrichum species are destructive pathogens of soybean causing anthracnose disease in pre- and post-harvest stages. Colletotrichum truncatum, which is associated with soybean anthracnose, was isolated from four geographical locations of India. Isolates were identified and grouped on the basis of rDNA internal transcribed spacer (ITS1 and ITS2) sequences. Multiple sequence alignment showed that one isolate had nucleotide variation at two positions, indicating genetic variability within C. truncatum. A maximum likelihood phylogenetic tree constructed for all the isolates indicated high sequence similarity of C. truncatum isolates. This study identified new C. truncatum isolate associated with soybean anthracnose which is different from the previously reported C. truncatum isolates.