Influence of some biotic and abiotic inducers on Fusarium wilt disease incidence of lupin (Lupinus albus) on disease resistance and protein pattern

摘要

Two biotic inducers (Pseudomonas fluorescens and P. putida) and three abiotic inducers (copper sulphate, indole butyric acid and potassium chloride) were tested for their ability to induce resistance in lupin plants against wilt disease caused by Fusarium oxysporum f. sp. lupini. Application of the inducers, as seed treatment, significantly reduced wilt disease incidence under greenhouse conditions. Potassium chloride and Pseudomonas fluorescens were superior. SDS- PAGE analysis of lupin seedlings revealed that seed treated with biotic and abiotic inducers resulted in a rapid induction of different novel PR-protein in shoot and root of lupin seedlings upon infection with the pathogen. These new proteins were not detected in untreated healthy or infected control seedlings.