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Analysis of genetic diversity and phylogeny of partial coat protein domain in Czech and Italian GFLV isolates

机译:捷克和意大利GFLV分离株的部分外壳蛋白结构域的遗传多样性和系统发育分析

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The genetic diversity of Grapevine fanleaf virus (GFLV) was evaluated in 4 isolates sampled from naturally infected grapevines from South Moravia (Czech Republic) and 2 Italian isolates from Bari (Italy). Conserved regions within sequences in databases were found and new primers corresponding to these regions were designed and tested for RT-PCR amplification of the CP codifying region. After sequencing of obtained amplicons the similarity of isolates was analysed via alignments of sequences and by means of dendrograms
机译:在从南摩拉维亚(捷克共和国)的自然感染葡萄树中取样的4种分离株和从巴里(意大利)的2种意大利分离株中,对葡萄扇叶病毒(GFLV)的遗传多样性进行了评估。发现数据库中序列内的保守区,并设计和测试对应于这些区的新引物,以用于CP编码区的RT-PCR扩增。对获得的扩增子进行测序后,通过序列比对和树状图分析分离物的相似性

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