Cytological investigations of the in vitro plants of Sedum telephium ssp. maximum L. at the root apex

摘要

While analyzing the results regarding the reaction of plant inoculum of Sedum telephium ssp. maximum L. produced by the propagules cultivated in the medium of Murashige - Skoog (1962) (MB - MS) with an input of different growth regulators, which stood at the basis of the experiments carried out within the monitorisation of the reactions of the explants of Sedum telephium ssp. maximum L., at the presence in the cultivation sub-layer that we modified, we have noticed -at the root apexes of the plants cultivated in MB – MS mediums with input of different growth regulators, using 1,5 mg/l from each of them-, a raspberry-redcoloration of the apexes of the small roots regenerated at the level of different types of explants, either propagules, or callus regenerated from the propagules. These particular reactions observed at the level of plant inoculum of Sedum telephium ssp. maximum L. determined us to lead our investigations towards the examination of these cells with an optical microscope. After the examination with an optical microscope of the root apexes of the in vitro plants of Sedum telephium ssp. maximum L. or of the small roots regenerated at the level of callus, we inferred the fact that, both the calyptra cells and the meristem (situated under the apex) have been coloured in red, especially in the case of the plant inoculum cultivated in the MB – MS medium, with a supplement of the mixture KIN and ANA (1,5mg/l from each of them). We highlighted the presence of these anthocyanins only at the level of plant inoculum of Sedum telephium ssp. maximum L., as this interesting phenomenon was not observed in the case of the organs belonging to plants cultivated in natural conditions. The presence of anthocyanins in the root apexes is a novelty in the specialty literature.