Determination of the capacity of absorption of the neutral red vital dye in the embryonic and secondary roots of maize (Zea mays) seedlings

摘要

To have valuable experimental results and to have the possibility to ensure the repeatability of this research, as experimental model we used seedlings, respectively their roots, especially the embryonic ones. The device with which the amount of neutral red was determined was a Spekol spectrophotometer. The determination of the concentration of neutral red solution was done using a standard curve which was “constructed” using the photometric data obtained by reading solutions of different concentrations of neutral red. From the physiological point of view, the method of staining of seedling tissue, or their organs, with a neutral red solution (prepared in tap water) can be regarded as being used as a method of evaluating the capacity of absorption of a compound and the reaction of certain living organs and tissues, according to the external conditions, more or less harmful, in comparison with similar cells or organs located in an optimal environment, taken as a control group.