Contraction mode itself does not determine the level of mTORC1 activity in rat skeletal muscle

摘要

Resistance training with eccentric contraction has been shown to augment muscle hypertrophy more than other contraction modes do (i.e., concentric and isometric contraction). However, the molecular mechanisms involved remain unclear. The purpose of this study was to investigate the effect of muscle contraction mode on mammalian target of rapamycin complex 1 (mTORC1) signaling using a standardized force?￠????time integral (load (weight)????????????contraction time). Male Sprague?￠????Dawley rats were randomly assigned to three groups: eccentric contraction, concentric contraction, and isometric contraction. The right gastrocnemius muscle was exercised via percutaneous electrical stimulation?￠????induced maximal contraction. In experiment 1, different modes of muscle contraction were exerted using the same number of reps in all groups, while in experiment 2, muscle contractions were exerted using a standardized force?￠????time integral. Muscle samples were obtained immediately and 3????h after exercise. Phosphorylation of molecules associated with mTORC1 activity was assessed using western blot analysis. In experiment 1, the force?￠????time integral was significantly different among contraction modes with a higher force?￠????time integral for eccentric contraction compared to that for other contraction modes ( P ????