EFEK FRAKSI ETIL ASETAT EKSTRAK ETANOL AKAR PASAK BUMI (Eurycoma longifolia, Jack) TERHADAP AKTIVITAS FAGOSITOSIS MAKROFAG SECARA IN VITRO

摘要

Eurycoma longifolia has been used as a remedy for detoxification, aphrodisiaca, anticancer and immunistimulant. The quassinoids of pasak bumi root extract, have antioxidant activity and immunomodulatory activity by increasing IL-12. This study aims to determine the effect of the ethyl acetate fraction of ethanolic extract of pasak bumi root on macrophages phagocytic activity in vitro. Macrophages were isolated from peritoneal fluid of male Balb/Cmice, and thenwere cultured for 24 hours in 24 well plate. The ethyl actetate fraction ethanolic extract of pasak bumi root with concentration of 10, 50, and 100 μg/ml, lipopolysaccharides and dimethyl sulfoxide were added, and incubated for 1.5 hours. The phagocytosis tests was carried out by adding latex with density 5x104 /well and incubated for 1.5 hours at incubator (5% CO2,37o C). Macrophage were then stained by Giemsa. The results showed that the percentage of active phagocytic cells treated with ethyl actetate fraction of ethanolic extract of pasak bumi root of 10, 50 and 100 μg/ml, lipopolysaccharides, and dimethyl sulfoxide were 95.4%, 90%, 85.4%, 83.4%, and 79% respectively. Phagocytic capacity at treatment levels of 10, 50, and 100 μg/ml, lipopolysaccharides,and dimethyl sulfoxide were 469; 439.8; 360.8; 204.6; and 147.6 respectively. The result showed thatreatment of ethyl acetate fraction of ethanolic extract of pasak bumi root can increase the phagocytic activity of macrophages in vitro.