New Derivative and Differential Spectrophotometric Methods for the Determination of Pterostilbene-An Antioxidant

摘要

Pterostilbene found in blueberries and Pterocarpus marsupium heartwoodis structurally similar to resveratrol. It is used for the treatment of cancer,diabetes and fungal infections. Three spectrophotometric methods havebeen developed for the determination of Pterostilbene. Two first derivativemethods and one difference spectrophotometric method were attempted.Two first derivative spectroscopic methods were developed in sodiumhydroxide (Method A) and methanol (Method B) and differential spectroscopicmethod (Method C) was developed using borate buffer and sodium hydroxide.Linearity was observed over the concentration range 0.1-25 and 1-25 μg/mlwith linear regression equations y = 0.0043x-0.0003 (R2=0.9997) andy=0.0037x - 0.0007 (R2=0.9997) for method A and B respectively whereasfor method C the linearity was followed over the concentration range 0.1-20 μg/ml with linear regression equation y=0.1124x - 0.0016 (R2=0.9999).The three methods were validated and can be applied for the determinationof Pterostilbene pharmaceutical formulations.