DETERMINA??O DOS LIMITES DE DETEC??O (LD) E QUANTIFICA??O (LQ) EM ANáLISE DE RESíDUOS DE PESTICIDAS ORGANOHALOGENADOS POR CROMATOGRAFIA EM FASE GASOSA

摘要

The detection (DL) and quantification limits (QL) of methods for pesticide residues analysis in a determined matrix are essential for the establishment of analytical capacity in qualitative and quantitative determination of traces of chemicals substances inherent. The determination of these limits in a matrix requires conducing experimental assays using concentration levels of DL and QL that reach the sensibility of the method. In pesticide residues analysis in food, the maximum residues limits (LMR) established for each culture by ANVISA (mg/kg of matrix weight), can constitute an initial parameter. In this study, a methodology to determinate these limits in apple were presented for the following organohalogenate pesticides: Chlorothalonil, Captan, Dicofol, Endosulfan I, Endosulfan II, Endosulfan sulfate and Vinclozolin. The results of DL and QL of the used method for accuracy (RM%= 64% to 127%) and precision (CV%= 2.11% to 12.55%), are within the values found in literature to consider such parameters as validated. Besides, the used methodology demonstrated capacity to reach values of QL and DL in concentrations below the recommended by brazilian legislation with the utilization of alternative analytical techniques (GC/ECD/ECD) to confirm the results.