Accumulation and biodistribution of gold nanoparticles in the mesenteric lymph nodes at oral administration

摘要

The. aim:.to. conduct. analysis. of. the. accumulation. and. biodistribution. of. gold. nanoparticles.in. the. structural. and. functional. areas.of. mesenteric. lymph. nodes. of. white. rats. conducted. with. regard. to. the. size. and. duration. of. the. oral. administration.. The. methods. of. dark.field. microscopy. with. Leica. DM. 2500. microscope. were. used. to. assess. biodistribution. of. gold. nanoparticle.. The. histochemical. reaction. of.silver. amplification. with. silver. lactate. by. Danscher. was. used. to. confirm. its. identification.. Results:. It. was. found. that. the. gold. nanoparticles.of. 1?3. nm. were. not. detected. by. these. methods. in. any. of. the. areas. of. the. lymph. nodes. regardless. of. the. duration. of. administration,. gold.nanoparticles. of. sizes.15.and.50.nm. were. detected.in. the. form. of.clusters. in. the.cytoplasm.of. macrophages.and. lymphocytes. mainly. in. the.mantle. zone. of. lymphoid. follicles. and. brain.strands..Conclusion:. It. was.established. that. the. gold.nanoparticles. of. sizes. 15. and. 50. nm. were.detected.in. the. form.of.clusters.in. the.cytoplasm.of.macrophages. and. lymphocytes