Combined effect of cytokinin and sucrose on in vitro tuberization parameters of two cultivars i.e. diamant and red norland of potato (Solanum tuberosum)

摘要

The present study is focused on the optimization of culture media and explant density of apical and nodal segments of two potato cultivars for In vitro microtuber production. Fifty media manipulations of Murashige & Skoog (1962) were tested by varying concentrations of sucrose (4, 6, 8, 10 and 12%) either alone on in combination (5-8% sucrose) with kinetin and BA. Different parameters with regard to microtuber induction mean number of microtubers, mean fresh weight of microtubers both from single and multinode explant of Diamant and Red Norland were studied. Medium MB8 (5mg BA and 8% sucrose) in term of induction and mean number of microtubers per single node explants and MB11 (6mg BA and 7% sucrose) in terms of mean fresh weight per single node provided best results for cv. Diamant. In case of Red Norland, medium MK10 (2mg Kinetin and 6% sucrose) in term of induction, mean number and mean fresh weight of microtubers per multinode explants gave best results. In comparison, cv. Diamant was better for microtuber induction and development than cv. Red Norland.