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Epidemiology of Citrus tristeza virus in nursery blocks of Citrus macrophylla and evaluation of control measures

机译:大叶柑橘苗圃中柑橘柑橘的流行病学及防治措施评价

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The control of Citrus tristeza virus (CTV), the causal agent of tristeza disease, is essential to guarantee a productive citrus industry. The specific features of a nursery block make the control of the natural spread of viruses transmitted by vectors more difficult. Thus, the knowledge of the epidemiology of CTV in nursery blocks is basic to the design control strategies of the disease. Two experimental nursery plots of alemow (Citrus macrophylla) were planted in open field in Moncada (Valencia, Spain) (with a high CTV prevalence, 85%), and in Alcanar (Tarragona, Spain) with 700 and 843 nursery plants, respectively. The plants were analysed by tissue print-ELISA using 3DF1 and 3CA5 CTV-specific monoclonal antibodies. The CTV prevalence estimated after one year of cultivation in both plots was of 24.37% and 3.91%, respectively. At the same time, a commercial nursery plot grown under plastic-net covers established in Alcanar with 1,200 plants was also analysed as above, being the CTV prevalence estimated of 0.00%. The aphid activity present in the nursery plots was monitored. Aphis spiraecola was the most abundant aphid species visiting the plants in the nursery plots grown in the open field, whereas the aphid activity registered in the plants grown under the plastic-net covers was practically zero. The percentage of individual aphids carrying CTV PCR-amplifiable targets detected by squash real-time RT-PCR in the open-field nursery plots was 17.5% in Moncada and 1.67% in Alcanar. No significant differences in the CTV prevalence between treated and non-treated plants with horticultural mineral oils were found in the area with the high CTV-inoculum pressure.
机译:防治Tristeza病的病原体Citris tristeza病毒(CTV)对于确保生产性柑橘产业至关重要。苗圃区的特定功能使控制由媒介传播的病毒的自然传播更加困难。因此,对育婴区CTV的流行病学知识是该疾病设计控制策略的基础。在蒙卡达(西班牙瓦伦西亚)(CTV患病率高达85%)和阿尔卡纳尔(西班牙塔拉戈纳)的空地上分别种植了700个苗圃和843个苗圃的两个试验性苗圃田(柑桔)。使用3DF1和3CA5 CTV特异性单克隆抗体通过组织印记-ELISA分析植物。两种土地栽培一年后估计的CTV患病率分别为24.37%和3.91%。同时,如上分析了在Alcanar建立的具有1200株植物的塑料网覆盖下生长的商业苗圃地,CTV流行率估计为0.00%。监测苗圃中存在的蚜虫活性。在空地上种植的苗圃地中,螺旋蚜是最丰富的蚜虫物种,而在塑料网覆盖下生长的植物中记录的蚜虫活性几乎为零。在露天保育园中,通过壁球实时RT-PCR检测到的携带CTV PCR可扩增靶标的单个蚜虫的百分比在蒙卡达州为17.5%,在阿尔卡纳尔地区为1.67%。在CTV菌体压力高的地区,未处理的园艺植物油与未处理的植物之间的CTV流行率无显着差异。

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