The effects of Metarhizium guizhouense PSUM02, petroleum oil, and Azadirachta excelsa seed kernels extract against Zeugodacus cucurbitae

摘要

Zeugodacus cucurbitae is a serious agricultural pest of cucurbitaceous crops. Metarhizium guizhouense PSUM02,petroleum oil, and Azadirachta excelsa seed kernel extract singly and combined were investigated for control of this pest. Theangled luffa fruit treated with petroleum oil and A. excelsa, or their mixtures showed egg-laying inhibition by 45.3-77.1%.Individually M. guizhouense PSUM02 inhibited egg-laying by 25.4%. The mixed application of M. guizhouense PSUM02 and A.excelsa had negative impacts on the number of eggs and larvae of Z. cucurbitae. In field conditions, the average fruit numbersand fruit weights of un-infested angled luffa indicated efficacy similar to malathion for treatment with M. guizhouense PSUM02+ petroleum oil and for M. guizhouense PSUM02 + petroleum oil + A. excelsa. The combined application of M. guizhouensePSUM02 with A. excelsa or petroleum oil showed negative effects to egg and larval stages of Z. cucurbitae providing analternative strategy for Z. cucurbitae control.