A Rapid Modified Antibody-Capture Enzyme-Linked Immunosorbent Assay (ELISA) for Detection HSV-1 Primary Infection in Human

M Ravanshad; MH Roustai; A Mostafaee; F Sabahi; H Soleimanjahi; M Ziyaeyan

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A Rapid Modified Antibody-Capture Enzyme-Linked Immunosorbent Assay (ELISA) for Detection HSV-1 Primary Infection in Human

机译：快速修饰的抗体捕获酶联免疫吸附测定（ELISA），用于检测人的HSV-1原发感染。

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Anantibody-captureenzyme-linkedimmunosorbentassay(ELISA)basedonthereactionbetweenantihumanIgMandserumIgMandHSV-1conjugatedwithhorseradishperoxidase(HRP)wasdeveloped.ThespecificityandsensitivityofthedevelopedHSV-1IgMELISAwereevaluatedandcomparedwithacommerciallyavailabletestkitbyusingsetsofwelldefinedseraofknownHSV-1primaryinfection.Forvalidation,526serumsampleshavebeentested.TheHSV-1IgMELISAhadacceptablesensitivityandspecificity,whichwerecomparablewiththatofthecommercialtestkit.Bothassaysshowedsomecross-reactivitywithseracontaininganti-HSV-2antibodies

机译：Anantibody-captureenzyme-linkedimmunosorbentassay（ELISA）basedonthereactionbetweenantihumanIgMandserumIgMandHSV-1conjugatedwithhorseradishperoxidase（HRP）wasdeveloped.ThespecificityandsensitivityofthedevelopedHSV-1IgMELISAwereevaluatedandcomparedwithacommerciallyavailabletestkitbyusingsetsofwelldefinedseraofknownHSV-1primaryinfection.Forvalidation，526serumsampleshavebeentested.TheHSV-1IgMELISAhadacceptablesensitivityandspecificity，whichwerecomparablewiththatofthecommercialtestkit.Bothassaysshowedsomecross-reactivitywithseracontaininganti HSV 2antibodies

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《Shiraz E Medical Journal》 |2006年第2期|共页
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M Ravanshad; MH Roustai; A Mostafaee; F Sabahi; H Soleimanjahi; M Ziyaeyan;
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中图分类基础医学;
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1. Establishment of an Algorithm Using prM/E- and NS1-Specific IgM Antibody-Capture Enzyme-Linked Immunosorbent Assays in Diagnosis of Japanese Encephalitis Virus and West Nile Virus Infections in Humans [J] . Jedhan U. Galula, Gwong-Jen J. Chang, Shih-Te Chuang, Journal of Clinical Microbiology . 2016,第2期

机译：建立使用prM / E-和NS1特异性IgM抗体捕获酶联免疫吸附测定法诊断人类乙型脑炎病毒和西尼罗河病毒感染的算法
2. PERFORMANCE OF COMMERCIAL ENZYME-LINKED IMMUNOSORBENT ASSAYS (ELISAS) FOR DIAGNOSIS OF HSV-1 AND HSV-2 INFECTION IN A CLINICAL SETTING [J] . Agyemang Elfriede, Warren Terri, Selke Stacy, Sexually transmitted diseases . 2016,第Suppla2期

机译：商业性酶联免疫吸附试验（ELISA）在诊断HSV-1和HSV-2感染中的临床表现
3. Kinetics of specific immunoglobulins M, E, A, and G in congenital, primary, and secondary cytomegalovirus infection studied by antibody-capture enzyme-linked immunosorbent assay. [J] . S L Nielsen, I S?rensen, H K Andersen Journal of Clinical Microbiology . 1988,第4期

机译：通过抗体捕获酶联免疫吸附试验研究了先天性，原发性和继发性巨细胞病毒感染中特定免疫球蛋白M，E，A和G的动力学。
4. PORCINE CIRCOVIRUS TYPE 2 : ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR THE DETECTION OF ANTIGEN AND ANTIBODIES IN FECES [C] . P Lopez, E Deshaies, N Brajon, Congress of the International Pig Veterinary Society . 2002

机译：猪圆环病毒2型：用于检测粪便中的抗原和抗体的酶联免疫吸附试验（ELISA）
5. Development of a monoclonal antibody-based antigen detection enzyme-linked immunosorbent assay (ELISA) for the diagnosis of human toxoplasmosis. [D] . Grushka, Daniel. 2001

机译：基于单克隆抗体的抗原检测酶联免疫吸附测定（ELISA）的开发，用于诊断人类弓形虫病。
6. Establishment of an Algorithm Using prM/E- and NS1-Specific IgM Antibody-Capture Enzyme-Linked Immunosorbent Assays in Diagnosis of Japanese Encephalitis Virus and West Nile Virus Infections in Humans [O] . Jedhan U. Galula, Gwong-Jen J. Chang, Shih-Te Chuang, 2016

机译：建立使用prM / E-和NS1特异性IgM抗体捕获酶联免疫吸附测定法诊断人类乙型脑炎病毒和西尼罗河病毒感染的算法
7. Establishment of an Algorithm Using prM/E- and NS1-Specific IgM Antibody-Capture Enzyme-Linked Immunosorbent Assays in Diagnosis of Japanese Encephalitis Virus and West Nile Virus Infections in Humans [O] . Jedhan U. Galula, Gwong-Jen J. Chang, Shih-Te Chuang, 2016

机译：使用PRM / E和NS1特异性IgM抗体 - 捕获酶联免疫吸附试验在人类中诊断中的鉴定酶联免疫吸附测定的建立
8. Indirect Immunoglobulin G (IgG) and IgM Enzyme-Linked Immunosorbent Assays (ELISAs) and IgM Capture ELISA for Detection of Antibodies to Lipopolysaccharide in Adult Typhoid Fever Patients in Pakistan. [R] . Sippel, J., Bukhtiari, N., Awan, M. B., 1989

机译：间接免疫球蛋白G（IgG）和Igm酶联免疫吸附试验（ELIsas）和Igm捕获ELIsa检测巴基斯坦成年伤寒患者脂多糖抗体。

A Rapid Modified Antibody-Capture Enzyme-Linked Immunosorbent Assay (ELISA) for Detection HSV-1 Primary Infection in Human

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