Primers microssatélites validados em tilápia do nilo no gene da aromatase cerebral (Cyp19b)

摘要

Aromatase cytochrome P450 is an enzyme that is responsible for a key step in the biosynthesis of estrogens, which is linked to reproduction. This enzyme is a product of the gene Cyp19 and catalyzes the formation of estrogen from androgen. Estrogen is essential for the development of the gonads and various other physiological processes, ranging from normal growth to reproductive behavior. This study aimed at validation of primers for microsatellite locus in the regulatory region of gene Cyp19b in Oreochromis niloticus. Thirty samples of three strains were analyzed: Chitralada and Supreme from larviculture Aquabel, and GIFT from the State University of Maringá (Paraná, Brazil). After extraction of DNA, the samples were subjected to PCR using primers designed to flank a microsatellite region. After confirmation of amplification, the samples were subjected to electrophoretic separation in the apparatus Origins (Elchrom Scientific, USA) to identify the alleles. A polymorphism in the regulatory region of Cyp19b was identified in the three strains. Three alleles, ranging from 123 to 141 bp, were observed. The primers for amplification of microsatellite in the regulatory region of gene Cyp19b were efficient and, therefore, they may be used for identification of mutations in this region in typical microsatellite analysis. Studies are being conducted to correlate the effect of temperature with the alleles observed in the animals sampled and the resulting sex ratio.