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Low temperature storage of NAA, GA3 and 2,4-D treated citrus budsticks

机译:NAA,GA3和2,4-D处理的柑橘芽条的低温储存

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摘要

Cold storage allows citrus budstick conservation for several months, although gradual bud viability loss and abscission of leaf petioles is observed. This study aimed to maintain bud viability reducing leaf petiole abscission in budsticks of 'Baianinha' and 'Valencia' sweet oranges (C. sinensis Osbeck), 'Tahiti' lime (C. latifolia Tanaka), and 'Murcott' tangor (C. sinensis × C. reticulata), using NAA, GA3, 2,4-D, and low temperature storage. After a 15-minutes-immersion in distilled water and NAA and GA3 solutions at concentrations of 10, 100, 500 and 1000 μmol L-1, and 2,4-D solutions at concentrations of 1, 10, 50 and 100 μmol L-1, the shoots were stored in polyethylene bags at 5 ± 1oC for 74 days. Percentage of leaf petiole abscission, fresh and dry matter losses, bud viability, and growth flush were evaluated. NAA and 2,4-D had the highest efficiency for petiole abscission inhibition, reaching almost 99% of success. The packaging system and shoot storage under low temperature efficiently reduced fresh and dry matter losses. Bud viability of 'Baianinha', 'Valencia' and 'Murcott' shoots was not reduced by storage, maintaining above 95% of grafting success. NAA, GA3 and 2,4-D treatments had no influence on sprout growth.
机译:冷藏可以使柑橘的芽杆保存数月,尽管观察到芽的活力逐渐下降和叶柄脱落。这项研究旨在维持芽的活力,降低“ Baianinha”和“ Valencia”甜橙(C. sinensis Osbeck),“ Tahiti”石灰(C. latifolia Tanaka)和“ Murcott” tangor(C。sinensis)的芽杆中的叶柄脱落。 ×C. reticulata),使用NAA,GA3、2,4-D和低温储存。浸入浓度为10、100、500和1000μmolL-1的蒸馏水和NAA和GA3溶液中以及浸入浓度为1、10、50和100μmolL-的2,4-D溶液中15分钟后如图1所示,将枝条放在5±1oC的聚乙烯袋中保存74天。评估了叶柄脱落的百分比,新鲜和干物质损失,芽活力和生长冲洗。 NAA和2,4-D抑制叶柄脱落的效率最高,几乎达到成功的99%。包装系统和芽的低温储存可有效减少新鲜和干物质的损失。贮藏不会降低'Baianinha','Valencia'和'Murcott'芽的芽活力,保持了超过95%的嫁接成功率。 NAA,GA3和2,4-D处理对新芽生长没有影响。

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