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Efeito do CPP-ACP sobre o esmalte erodido extrinsecamente: estudo in vitro

机译:CPP-ACP对外在腐蚀牙釉质的影响:体外研究

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Objective: The aim of this study was to evaluate the remineralization effect of CPP-ACP on tooth enamel that is eroded extrinsically. Methods: Sixty freshly extracted bovine teeth were stored in a solution of thymol (0.1%), cut into enamel blocks (6x6mm), abraded with silicon carbide sandpaper (600, 800 and 1200 grit) and polished with felt. The specimens were randomly divided into 4 groups (n=15), varying the immersion medium , deionized water or citric acid, and the optional use of CPP-ACP. (G1: deionized water; G2: citric acid; G3: deionized water + CPP-ACP; G4: citric acid + CPP-ACP). The samples were subjected to 4 cycles of erosion every day, comprising 5 minutes immersion in one medium and 2 hours of immersion in artificial saliva, for 5 consecutive days. After each erosive cycle, 20 uL of the MI Paste was applied for 5 minutes on one half of the samples and washed with deionized water. Before and after the erosive cycling, the measurements of Vickers hardness and surface roughness were taken. Results: The results were evaluated through statistical testing. The final hardness and roughness values showed no statistically significant difference between G2 and G4. The paste in G4 did not promote an increase in hardness values nor a decrease in roughness values. Conclusion: It was concluded that CPP-ACP was unable to remineralize enamel subjected to extrinsic erosion, being ineffective in preventing erosion of enamel and in the remineralization of the eroded enamel.
机译:目的:本研究的目的是评估CPP-ACP对外在侵蚀的牙釉质的再矿化作用。方法:将60颗新鲜提取的牛牙齿储存在百里酚(0.1%)的溶液中,切成搪瓷块(6x6mm),用碳化硅砂纸(600、800和1200粗砂)擦净,并用毛毡抛光。将标本随机分为4组(n = 15),改变浸没介质,去离子水或柠檬酸,以及可选地使用CPP-ACP。 (G1:去离子水; G2:柠檬酸; G3:去离子水+ CPP-ACP; G4:柠檬酸+ CPP-ACP)。每天对样品进行4次腐蚀循环,包括连续5天浸入一种介质中5分钟和浸入人工唾液中2小时。在每个侵蚀周期后,将20 uL的MI Paste涂在一半的样品上5分钟,然后用去离子水洗涤。在侵蚀循环之前和之后,进行维氏硬度和表面粗糙度的测量。结果:通过统计学测试评估结果。最终的硬度和粗糙度值在G2和G4之间没有统计学上的显着差异。 G4中的糊剂没有促进硬度值的增加或粗糙度值的降低。结论:结论是CPP-ACP不能使遭受外在腐蚀的牙釉质再矿化,不能有效防止牙釉质的腐蚀和侵蚀的牙釉质的再矿化。

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