Expression Pattern of the Orphan Nuclear Receptor, Nurr1, in the Developing Mouse Forelimb and its Relationship to Limb Skeletogenesis and Osteogenesis | Science Publications

摘要

> class="block-title sub-title" style="text-align:justify">Abstract: The NR4A orphan nuclearreceptor, Nurr1, has been shown to regulate the expression of osteoblasticgenes and osteoblastic differentiation. However, the expression profile ofNurr1 in the developing mouse forelimb and its relationship to skeletogenesishas not, to the best of our knowledge, been previously analyzed. In this study,the relationship between Nurr1 expression pattern, skeletogenesis andosteogenesis in the developing mouse forelimb was investigated. The expressionlevel of Nurr1 during development was also quantified by real time-polymerase chainreaction. Our results revealed that Nurr1 is expressed in the mesenchyme cellsthat will form the skeleton. Nurr1 is aabundantly expressed in the primaryossification centers of the forelimb skeletal elements and its expression levelis gradually increased during limb development, particularly, at the onset ofossification. Collectively, these data suggested that Nurr1 plays an importantrole in skeletogenesis and patterning of the developing mouse forelimb.