Rapid species identification of Candida directly from blood culture broths by Sepsityper-MALDI-TOF mass spectrometry: impact on antifungal therapy

摘要

Method We applied Sepsityper matrix-assisted laser desorption ionisationtime of flight mass spectrometry (MALDI-TOF-MS) technology on positive blood culture broths for rapid species identification. Results Of the 74 patients with candidaemia, 25 had the species identified on the day of the positive blood culture directly from the broth (rapid identi cation group) while the remaining 49 had the species identi ed from culture (conventional identi cation group). Three (13.6%) out of 22 treated patients in the rapid identi cation group received echinocandin compared to 20/45 (44.4%) in the conventional identi cation group. The appropriateness of therapy was 90.9% in the rapid identi cation group and 62.2% in the conventional identi cation group (p = 0.01). Cost savings were more than ￡10,000 in the first three days of treatment. Conclusion Sepsityper-MALDI-TOF-MS is a useful tool in supporting antifungal stewardship programmes.