Utilization of dairy effluent as alternative fermentation medium for microbial lipase production

摘要

Annually, dairy industries produce a lot of crude materials as waste that are difficult to treat and valorize. Using dairy effluent as fermentation medium, this study aiming to optimize the lipase production by Trichoderma atroviride 676. The experimental mixture design (EMD) was adopted to optimize the enzyme production by the supplementation of effluent with tween 80, olive oil and Vogel’s medium. According to the results, the optimum medium containing 5 ml/ of olive oil, 5.62 ml/l of Tween 80, 15 ml/ of Vogel’s medium led to a maximum lipase production of 1327.28 U/ml, which was 123-fold higher that the dairy effluent without supplementation. These data clearly demonstrates the possibility of using dairy effluent as a potential fermentation medium for production of lipases. The search for alternatives substrates for lipase production can contribute in getting lipases with a reduction of the production cost in industrial scale, and help to reduce the massive environmental impact caused by dairy industries.