Genetic diversity and phylogenetic relationships of Romanian cattle breeds inferred from cytochrome b gene partial sequences

摘要

To determine the origin and genetic diversity of Romanian cattle breeds: Romanian Grey Steppe, Romanian Black Spotted, Romanian Brown and Montbeliarde, the partial Cyt b gene sequences (610bp) were analyzed. The average nucleotide frequencies of T, C, A and G were 27.3, 27.6, 30.9 and 14.2%, respectively. The nucleotide composition is rich in A/T nucleotides and shows a slightly higher A/T content (58.2%) than C/G content (41.8%). The nucleotide comparison also demonstrated a strong bias towards transition. A Neighbor-Joining tree was constructed and it revealed that the Romanian cattle breeds originated from Bos taurus and had no direct relationship with Bos indicus, Bos grunniens and Bos javanicus.