Sol-Gel Entrapment of Bacillus licheniformis CMIT 1.33 Proteases in Silica-Gels

摘要

In the present work, the immobilization technique by entrapment in silica gels of Bacillus licheniformis CMIT 1.33 local strain proteases was studied. Enzymes entrapment in solid mesoporous silica gels obtained by sol-gel method was performed by in situ polymerization of tetraethoxisilane (TEOS) and tetramethoxisilane (TMOS). The native enzymatic preparation was entrapped at neutral pH, in presence of a pore forming agent. Entrapped enzymes in silica gels have been clearly shown to retain their biocatalitic activity and exhibit increased stability at environmental effects. The enzyme-substrate affinity modification was investigated based on the kinetics of native and immobilized enzymes respectively, in the hydrolysis of a model substrate with low molecular mass.