Biocompatibility Study of Several Esthetic Dental Restorative Materials

摘要

Biocompatibility of three materials used in restorative dentistry were investigated in vitro. The aim of this study was a comparative evaluation of biocompatibility of several restorative materials on cultures of primary human osteoblasts (OBL) and HFL (human lung fibroblasts) cells. The differentiation process was evaluated with alamar blue viability assay and by immunocytochemical staining. The samples were examined using a Zeiss Axiovert microscope by reversed phase fluorescence and image capture was performed using a MRC camera. A glass ionomer cement (Ketac Molar, 3M ESPE), a light cured universal microhybrid composite material (Fitek Z250, 3M ESPE) and a total etch adhesive (Adper Single Bond 2 Adhesive, 3M ESPE) were used for analysis. The results of this study have shown that none of these materials exerted increased toxicity, explained by the low capacity of these materials to allow cell adhesion. Only HFL cells grown on Adper Single Bond 2 showed some degree of adhesion to the substrate.