Efficacy of Light Emitting diodes (LEDs) Lighting System for In vitro Shoot Regeneration of Medicinal Water hyssop (Bacopa monnieri L. PENNEL)

摘要

Efficacy of different Light Emitting Diodes (LEDs) on in vitro whole plant regeneration of waterhyssop (Bacopa monnieri L. Pennel) was investigated in this study. Shoot tip explants were cultured onMS medium with BA (0.25, 0.50 and 1.0 mg l-1) and incubated under Red:Blue (R:B) LEDs in differentcombinations (4:1, 3:1, 2:1 and 1:1 R:B and white LEDs) for 10 weeks. Callus induction and shootregeneration was noted on all explants irrespective of LEDs type or BA concentration. Shoots perexplant and shoot length ranged 6.56-10.42 and 0.96-1.71cm respectively. Maximum number of 10.42shoots with highest shoot length of 1.71 cm was achieved under 1:1 R:B LEDs. Whereas, different BAconcentrations generated 4.97-11.98 shoots per explant with shoot length in the range of 0.93-1.35 cm.Maximum shoots per explant (10.35) with more longer shoots (1.31 cm) was achieved on MS mediumcontaining 1.0 mg l-1 BA. Comparing of BA × LEDs, maximum number of 15.17 shoots per explant wasinduced under 1.0 mg l-1 BA × 2:1 R:B LEDs. Whereas, longer shoots (2.68 cm) were scored under 0.5mg l-1 BA × 2:1 R:B LEDs. Regenerated shoots were rooted using 1.0 mg l-1 IBA in four weeks followedby acclimatization in the aquariums provided with tap water. Results revealed that LEDs incombination with BA can be successfully employed for multiple shoot regeneration of water hyssop.